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Blunt/TA Ligase Master Mix
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Blunt/TA Ligase Master Mix
Blunt/TA Ligase Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(25)
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New England Biolabs for
Blunt/TA Ligase Master Mix
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Company Info
Citations
Reviews
Product Specs
Item
Blunt/TA Ligase Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0367L
Quantity
250 reactions
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Enzymes
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (25)
(1)
Development of a novel streamlined workflow (AACRE) and database (inCREDBle) for genomic analysis of carbapenem-resistant Enterobacterales
Microbial Genomics
November 27, 2023
Tyler S. Alioto, Marta Gut, Bruno Kotska Rodiño-Janeiro, Fernando Cruz, Jèssica Gómez-Garrido, Juan Carlos Vázquez-Ucha, Caterina Mata, Regina Antoni, Ferran Briansó, Marc Dabad,
et al.
ligated 1 h at room temperature (RT) to the purified DNA using the
NEB
Blunt/TA ligase Master Mix
(
NEB
,
M0367L
) and purified with 1× AMPure XP Beads. Once purified, a 1 µg equimolar pool of the barcoded samples
(2)
Evolution of loss of heterozygosity patterns in hybrid genomes of Candida yeast pathogens
BMC Biology
May 11, 2023
Verónica Mixão, Juan Carlos Nunez-Rodriguez, Valentina del Olmo, Ewa Ksiezopolska, Ester Saus, Teun Boekhout, Attila Gacser, Toni Gabaldón
Adapter Mix (AMX1D, ONT) was ligated to the purified sample using the
Blunt/TA Ligase Master Mix
(
NEB
,
M0367L
). The adapter-ligated product was purified using 0.4-fold excess of AMPure XP beads. The beads were
(3)
Critically short telomeres derepress retrotransposons to promote genome instability in embryonic stem cells
Cell Discovery
May 2, 2023
Nannan Zhao, Guoxing Yin, Chun Liu, Weiyu Zhang, Yang Shen, Dan Wang, Zhenzhen Lin, Jiao Yang, Jian Mao, Renpeng Guo,
et al.
NEBNext Ultra II End Repair/dA-Tailing Module
(
E7546L
,
NEB
). Adapter
ligation
was performed using the NEBNext
Blunt/TA Ligase Master Mix
(
M0367L
,
NEB
) and
Ligation
Sequencing
Kit 1D (SQK-LSK109, Oxford Nanopore Technologies). DNA was purified at
(4)
An Insight into the Genome of Pathogenic and Non-Pathogenic Acanthamoeba
Pathogens
December 19, 2022
Chayan Sharma, Sumeeta Khurana, Amit Arora, Alka Bhatia, Amit Gupta
(Beckmann Coulter, Pasadena, CA, USA). Native barcode
ligation
was performed with NEB blunt/TA ligase (
NEB
#
M0367L
, New England Biolabs, Ipswich, MA, USA) and cleaned with 1x AmPure beads. Qubit-quantified barcode ligated
(5)
Million spot binding array platform for exploring and optimizing multiple simultaneous detection events
STAR Protocols
December 1, 2022
Michael Gibbons, Jessica M. Hong, Mikelle Foster, Mariya Chavarha, Shirley Shao, Llyke Ching, Victoria A. Church, Lauren Schiff, Sara Ahadi, Marc Berndl,
et al.
cycle),Illumina,MS-102-3001 PhiX Control v3,Illumina,FC-110-3001
Blunt/TA Ligase Master Mix
,
NEB
,Cat#
M0367L
SoluLINK
Protein-Oligonucleotide Conjugation Kit
,
Vector Laboratories
,
S-9011-1
,, Deposited data,Deposited
(6)
Benchmarking second and third-generation sequencing platforms for microbial metagenomics
Sci Data
November 11, 2022
Victoria Meslier, Benoit Quinquis, Kévin Da Silva, Florian Plaza Oñate, Nicolas Pons, Hugo Roume, Mircea Podar, Mathieu Almeida
barcode using Native Barcoding Expansion 1–12 kit (EXP-NBD104) and Blunt/TA Ligase (
New England Biolabs
,
M0367L
). Native barcode ligated DNA was quantified with Qubit and Fragment Analyzer. Equimolar libraries were
(7)
Simultaneous measurement of the size and methylation of chromosome 4qA-D4Z4 repeats in facioscapulohumeral muscular dystrophy by long-read sequencing
J Transl Med
November 8, 2022
Yosuke Hiramuki, Yuriko Kure, Yoshihiko Saito, Megumu Ogawa, Keiko Ishikawa, Madoka Mori-Yoshimura, Yasushi Oya, Yuji Takahashi, Dae-Seong Kim, Noriko Arai,
et al.
EXP-NBD104) were ligated to cleaved and dA-tailed genomic DNAs using
Blunt/TA Ligase Master Mix
(
NEB
, #
M0367L
) at room temperature for 10 min, followed by
purification
with Agencourt AMPure XP Beads (
Beckman Coulter
,
(8)
Chromosome-level assemblies from diverse clades reveal limited structural and gene content variation in the genome of Candida glabrata
BMC Biology
October 8, 2022
Marina Marcet-Houben, MarÃa Alvarado, Ewa Ksiezopolska, Ester Saus, Piet W. J. de Groot, Toni Gabaldón
Adapter Mix (AMX1D, ONT) was ligated to the purified samples using the
Blunt/TA Ligase Master Mix
(
NEB
,
M0367L
). When using the SQK-LSK308 kit, two adapter
ligation
steps were required. First, the 1D2 adapter was
(9)
Long-read sequencing unveils high-resolution HPV integration and its oncogenic progression in cervical cancer
Nature communications
May 10, 2022
Zhou L, Qiu Q, Zhou Q, Li J, Yu M, Li K, Xu L, Ke X, Xu H, Lu B,
et al
.
NEBNext Ultra II End Repair/dA-Tailing Module
(
E7546L
,
NEB
). Adapter
ligation
was performed using the NEBNext
Blunt/TA Ligase Master Mix
(
M0367L
NEB
) and a
Ligation
Sequencing
Kit 1D (SQK-LSK109). DNA was purified between each step using Agencourt
(10)
High throughput sequencing based direct detection of SARS-CoV-2 fragments in wastewater of Pune, West India
Science of The Total Environment
February 10, 2022
Tanmay Dharmadhikari, Vinay Rajput, Rakeshkumar Yadav, Radhika Boargaonkar, Dhawal Patil, Saurabh Kale, Sanjay P. Kamble, Syed G. Dastager, Mahesh S. Dharne
A63881
). End preparation and Barcoding was performed using Blunt/TA Ligase MasterMix (
New England Biolabs
;
M0367L
),
NEBNext Ultra II End Repair/dA-Tailing Module
(
New England Biolabs
;
E7546L
) and Native Barcoding Expansion
(11)
Monoclonal antibodies targeting two immunodominant epitopes on the Spike protein neutralize emerging SARS-CoV-2 variants of concern
eBioMedicine
February 1, 2022
Branislav Kovacech et al.
Barcoding Expansion 96 kit (EXP-NBD196, Oxford Nanopore Technologies) and a Blunt/TA
Ligation
Master Mix (
M0367L
,
New England Biolabs
). Barcoded samples (96) were pooled and purified using 0·5 volume of AMPure XP
(12)
ProtSeq: Toward high-throughput, single-molecule protein sequencing via amino acid conversion into DNA barcodes
iScience
January 21, 2022
Hong JM, Gibbons M, Bashir A, Wu D, Shao S, Cutts Z, Chavarha M, Chen Y, Schiff L, Foster M,
et al
.
cycle),Illumina,MS-102-3001 PhiX Control v3,Illumina,FC-110-3001
Blunt/TA Ligase Master Mix
,
NEB
,Cat#
M0367L
SoluLINK
Protein-Oligonucleotide Conjugation Kit
,
Vector Laboratories
,
S-9011-1
Pierce™ BCA Protein Assay Kit
(13)
Structural variants in the Chinese population and their impact on phenotypes, diseases and population adaptation
Nature Communications
December 1, 2021
Zhikun Wu, Zehang Jiang, Tong Li, Chuanbo Xie, Liansheng Zhao, Jiaqi Yang, Shuai Ouyang, Yizhi Liu, Tao Li, Zhi Xie
NEBNext Ultra II End Repair/dA-Tailing Module
(
E7546L
,
NEB
). Adapter
ligation
was performed using NEBNext
Blunt/TA Ligase Master Mix
(
M0367L
NEB
) and
Ligation
Sequencing
Kit 1D (SQK-LSK109, Oxford Nanopore Technologies). Library fragments with
(14)
Using Combined Methods of Genetic Mapping and Nanopore-Based Sequencing Technology to Analyze the Insertion Positions of G10evo-EPSPS and Cry1Ab/Cry2Aj Transgenes in Maize
Frontiers in Plant Science
July 29, 2021
Cheng Peng, Yingting Mei, Lin Ding, Xiaofu Wang, Xiaoyun Chen, Junmin Wang, Junfeng Xu
end-repair process, DNA was ligated to the
sequencing
adapter, using the
Blunt/TA Ligase Master Mix
(
M0367L
,
NEB
). Then, the barcoded DNA was purified again with AMPure XP beads. To
measure
the concentration
(15)
Genomic monitoring of SARS-CoV-2 uncovers an Nsp1 deletion variant that modulates type I interferon response
Cell host & microbe
March 10, 2021
Lin JW, Tang C, Wei HC, Du B, Chen C, Wang M, Zhou Y, Yu MX, Cheng L, Kuivanen S,
et al
.
A63880 Ethanol,Sangon,Cat# A500737 Isopropanol,Sangon,Cat# A507048 NEB
Blunt/TA Ligase Master Mix
,
New England Biolabs
,Cat#
M0367L
NEBNext End repair / dA-tailing Module,New England Biolabs,Cat# E7546 2 × SYBR Green Master Mix,
Bio-Rad Laboratories
(16)
人类肠道病毒粒子富集及纳米孔测序
BIO-PROTOCOL
January 1, 2021
佳宝 曹, 雨青 张, 娜 赵, 军 王
End-prep enzyme mix (NEB, catalog number: E7372AA) 19.
Blunt/TA ligase master mix
(
NEB
, catalog number:
M0367L
) 20. Quick
T4 DNA ligase
(
NEB
, catalog number: E6057AA) 21. 核酸染料 (Mei5bio, catalog number: MF079-plus-05) 22.
(17)
Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing
Frontiers in Veterinary Science
October 26, 2020
Emeli Torsson, Tebogo Kgotlele, Gerald Misinzo, Jonas Johansson Wensman, Mikael Berg, Oskar Karlsson Lindsjö
reactions),4.10 Native barcoding expansion 1-12,EXP-PBC001,Oxford Nanopore,USD 288*,4
Blunt/TA ligase master mix
,
M0367L
,
New England Biolabs
,USD 520 (250 reactions),20.80
Ligation
sequencing
kit (incl. FlowCell priming Kit),SQK-LSK109,Oxford
(18)
Comprehensive analysis of structural variants in breast cancer genomes using single-molecule sequencing
Genome Research
September 1, 2020
Sergey Aganezov et al.
ligated to Oxford Nanopore adapters (Oxford Nanopore SQK-LSK108) via the NEB blunt/TA master mix (
NEB
M0367L
), and cleaned up with Ampure beads (
Beckman Coulter
A63881
). The full volume of the prepared libraries
(19)
Whole-Genome Sequence of an Indian Group A
Streptococcus emm
Type 1-2 Strain Isolated from a Blood Sample in North India
Microbiology Resource Announcements
May 7, 2020
Vivek Sagar, Anuradha Chakraborti, Rajesh Kumar
(Beckmann Coulter, USA). Native barcode
ligation
was performed with a blunt/TA (
T4 DNA) ligase
mix (
M0367L
;
New England BioLabs
) using the native barcoding genomic DNA kit (EXP-NBD104; Oxford Nanopore Technologies,
(20)
Genomic Epidemiology of SARS-CoV-2 in Guangdong Province, China
Cell
May 1, 2020
Jing Lu, Louis du Plessis, Zhe Liu, Verity Hill, Min Kang, Huifang Lin, Jiufeng Sun, Sarah François, Moritz U.G. Kraemer, Nuno R. Faria,
et al.
NEBNext Ultra II End repair/dA-tailing Module
,
New England BioLabs
,Cat#
E7546L
NEB
Blunt/TA Ligase Master Mix
,
New England BioLabs
,Cat#
M0367L
NEBNext Quick Ligation Module
,
New England BioLabs
,Cat#
E6056L
TURBO DNase,Thermo Fisher,Cat# AM2239 MGIEasy
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Blunt/TA Ligase Master Mix from New England Biolabs
Supplier Page
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Pricing Info
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